Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production.

The data presented in this article relates to the manuscript entitled 'Engineering Chinese hamster ovary cell lipid metabolism results in an expanded and enhanced ER recombinant biotherapeutic protein production', published in the journal Metabolic Engineering [1]. 

In the article here, we present data check excess of modifying lipid metabolism and SREBF1 SCD1 gene in CHO cells by densitometry of Western E.coli Recombinant Proteins

 Blot and by using mass spectrometry to investigate the impact on a particular lipid species. We also immunofluorescence data present on the SCD1 protein levels and SREBF1 excess. 

Data growth profile during the batch culture and control CHO cells CHO cells engineered to overexpress SCD1 and SREBF1 during batch culture are also reported. Finally, we report data on the model results secretion of recombinant proteins produced from the control, SCD1 or SREBF1 engineered cells using a transient expression system.

Alanine Substitution Cross-React inactivate epitope on recombinant Dengue Virus Envelope Protein.

Expansion of mosquito habitats belonging to the genus http://bmp-5.com/ Aedes put nearly half of the world's population at risk of contracting dengue fever, and a significant fraction will develop serious hemorrhagic complications, which can be fatal if not properly diagnosed and treated in a timely fashion. Although several diagnostic methods have been approved for the diagnosis of dengue, its application is limited in rural areas in developing countries with the sample preparation costs and methodological requirements, as well as cross-reactivity between the different serotypes of the dengue virus and other flaviviruses, such as Zika virus.

 For this reason, it is necessary to generate more specific antigen to improve serological methods that could be cheaper and used in field operations. Here, we describe the strategy for inactivation epitope cross-react on the surface Dengue envelope proteins of the virus through the generation of synthetic peptide sequence recombinant, where the key amino acid residues of Dengue virus serotype 1 (DENV-1) and 2 (DENV-2) is replaced by residues alanine. 

Proteins thus produced is recognized by 88% of sera from Dengue NS1 + patients and showed an increase in serotype specificity because they do not react with antibodies present in seroconversion, PCR-serotyped DEN-4 infected patients.